Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242) (HY057043)

Name: Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242)
Brand: abinScience
SKU: HY057043
Price: 126 USD
Availability: InStock

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Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Изображения
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Даташит
SDS

Обзор

Каталожный номер

HY057043

Описание

Anti-Human CD3E Nanobody (SAA2242) [SAA2242] (HY057043) is a alpaca monoclonal antibody detecting CD3e in ELISA, FCM. Suitable for Human.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

Реактивность видов

Human

Применения

ELISA, FCM

Хозяинский вид

Alpaca

Клоональность

Monoclonal

Изотип

VHH-His

ID клона

SAA2242

Проверенные приложения

FCM

Цель

CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

Уровень эндотоксинов

< 10 EU/mg

Чистота

>95% as determined by SDS-PAGE.

Очистка

Purified by Nickel column.

Номер доступа

P07766

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4.

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Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto).

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1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938

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2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002

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3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

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4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325

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5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660

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Примечание

For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Anti-Human CD3E Nanobody (SAA2242)
Bioactivity

Detects recombinant human CD3E (Catalog No: HY057031) in indirect ELISA.
Flow cytometry

Flow cytometry analysis was performed using anti-human CD3 monoclonal antibody. Jurkat cells were incubated with an irrelevant antibody (blue histogram) or FITC-conjugated anti-human CD3 monoclonal antibody (Catalog # HY057043, green histogram) at a concentration of 5 µg/mL for 30 min at room temperature. After washing, cells were directly analyzed on a NovoCyte Flow Cytometer.