Anti-Human CD40/TNFRSF5 Recombinant Antibody (SAA2006) (HB782023)

Name: Anti-Human CD40/TNFRSF5 Recombinant Antibody (SAA2006)
Brand: abinScience
SKU: HB782023
Price: 110 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HB782023

Reactividad de especies

Human

Aplicaciones

ELISA, WB, FCM

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

SAA2006

Aplicaciones probadas

FCM

Objetivo

B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

Nivel de endotoxinas

< 10 EU/mg

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P25942

RRID

Anti-Human CD40/TNFRSF5 Antibody (SAA2006) (abinScience Cat# HB782023, RRID:AB_3727918)
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Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Tumor necrosis factor receptor superfamily member 5 (CD40/TNFRSF5) is a ~30 kDa protein. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

1. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973

Nota

For research use only.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Imágenes

Bioactivity

SEC-HPLC detection for Anti-Human CD40/TNFRSF5 Antibody (ABS2001).
Flow Cytometry

Flow-cytometry using anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD40 monoclonal antibody (Catalog HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD40/TNFRSF5 Antibody (SAA2006).
Flow CytoMetry

Flow-cytometry using APC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.