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| カタログ番号 | HB782023 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||
| アプリケーション | ELISA, WB, FCM | ||||||
| 宿主種 | Human | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG1, kappa | ||||||
| クローンID | SAA2006 | ||||||
| テスト済みアプリケーション | FCM | ||||||
| ターゲット | B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5 | ||||||
| エンドトキシンレベル | < 10 EU/mg | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | P25942 | ||||||
| RRID | Anti-Human CD40/TNFRSF5 Antibody (SAA2006) (abinScience Cat# HB782023, RRID:AB_3727918) |
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| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 背景 | Tumor necrosis factor receptor superfamily member 5 (CD40/TNFRSF5) is a ~30 kDa protein. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. 1. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973 | ||||||
| 注意事項 | For research use only. | ||||||
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SEC-HPLC detection for Anti-Human CD40/TNFRSF5 Antibody (ABS2001).
Flow-cytometry using anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD40 monoclonal antibody (Catalog HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human CD40/TNFRSF5 Antibody (SAA2006).
Flow-cytometry using APC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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