Anti-Human CD40/TNFRSF5 Recombinant Antibody (SAA2006) (HB782023)

Name: Anti-Human CD40/TNFRSF5 Recombinant Antibody (SAA2006)
Brand: abinScience
SKU: HB782023
Price: 110 USD
Availability: InStock

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Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HB782023

Реактивность видов

Human

Применения

ELISA, WB, FCM

Хозяинский вид

Human

Клоональность

Monoclonal

Изотип

IgG1, kappa

ID клона

SAA2006

Проверенные приложения

FCM

Цель

B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

Уровень эндотоксинов

< 10 EU/mg

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P25942

RRID

Anti-Human CD40/TNFRSF5 Antibody (SAA2006) (abinScience Cat# HB782023, RRID:AB_3727918)
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Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4.

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Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Tumor necrosis factor receptor superfamily member 5 (CD40/TNFRSF5) is a ~30 kDa protein. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

1. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973

Примечание

For research use only.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Изображения

Bioactivity

SEC-HPLC detection for Anti-Human CD40/TNFRSF5 Antibody (ABS2001).
Flow Cytometry

Flow-cytometry using anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD40 monoclonal antibody (Catalog HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD40/TNFRSF5 Antibody (SAA2006).
Flow CytoMetry

Flow-cytometry using APC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.