Anti-Human CD5 Recombinant Antibody (5D7), APC (HY046237)

Name: Anti-Human CD5 Recombinant Antibody (5D7), APC
Brand: abinScience
SKU: HY046237
Price: 154 USD
Availability: InStock

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Vista general

Número de catálogo	HY046237
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG1
ID de clon	5D7
Conjugación	APC
Aplicaciones probadas	FCM
Objetivo	Lymphocyte antigen T1/Leu-1, CD5, T-cell surface glycoprotein CD5, LEU1
Nivel de endotoxinas	Please contact with the lab for this information.
Productos asociados	Mouse IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Número de acceso	P06127
Forma	Liquid
Buffer de almacenamiento	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo	T-cell surface glycoprotein CD5 is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. 1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049 2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158 3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399 4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044
Nota	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using APC anti-human CD5 antibody. Jurkat cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-human CD5 antibody monoclonal antibody (Catalog # HY046237, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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Datasheet