Anti-Human CD5 Recombinant Antibody (H65), APC (HY046137)

Name: Anti-Human CD5 Recombinant Antibody (H65), APC
Brand: abinScience
SKU: HY046137
Price: 154 USD
Availability: InStock

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Каталожный номер	HY046137
Реактивность видов	Human
Применения	FCM, IF, WB
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	IgG1, kappa
ID клона	H65
Конъюгация	APC
Проверенные приложения	FCM
Цель	Lymphocyte antigen T1/Leu-1, CD5, T-cell surface glycoprotein CD5, LEU1
Уровень эндотоксинов	Please contact with the lab for this information.
Связанные продукты	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Номер доступа	P06127
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон	T-cell surface glycoprotein CD5 is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. 1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049 2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158 3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399 4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044
Примечание	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Изображения

Flow Cytometry

Flow-cytometry using APC anti-human CD5 antibody. Jurkat cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-human CD5 antibody monoclonal antibody (Catalog # HY046137, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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