Anti-Human CD5 Recombinant Antibody (H65), FITC (HY046117)

Name: Anti-Human CD5 Recombinant Antibody (H65), FITC
Brand: abinScience
SKU: HY046117
Price: 118 USD
Availability: InStock

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概要
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概要

カタログ番号	HY046117
種反応性	Human
アプリケーション	FCM, IF, WB
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	H65
抱合	FITC
テスト済みアプリケーション	FCM
ターゲット	Lymphocyte antigen T1/Leu-1, CD5, T-cell surface glycoprotein CD5, LEU1
エンドトキシンレベル	Please contact with the lab for this information.
関連製品	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), FITC
アクセッション番号	P06127
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	T-cell surface glycoprotein CD5 is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. 1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049 2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158 3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399 4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using FITC anti-human CD5 antibody. Jurkat cells were stained with an irrelevant antibody (Green Histogram) or a FITC anti-human CD5 antibody monoclonal antibody (Catalog # HY046117, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献

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