Anti-Human CD52 Recombinant Antibody (SAA0024) (HB894207)

Name: Anti-Human CD52 Recombinant Antibody (SAA0024)
Brand: abinScience
SKU: HB894207
Price: 196 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB894207
Reactivité des espèces	Human
Applications	FCM, WB
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	SAA0024
Applications testées	WB, FCM
Cible	Human epididymis-specific protein 5, CDW52, CAMPATH-1 antigen, Cambridge pathology 1 antigen, He5, HE5, Epididymal secretory protein E5, CD52, CDw52
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P31358
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	CAMPATH-1 antigen (CD52) is a ~6 kDa protein. May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human CD52 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD52 antibody monoclonal antibody (Catalog # HB894207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD52 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD52 monoclonal antibody (Catalog: HB894207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD52 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD52 (Catalog No: HB894011)
Lane 2: negative control