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| カタログ番号 | HB894207 |
|---|---|
| 種反応性 | Human |
| アプリケーション | FCM, WB |
| 宿主種 | Mouse |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1, kappa |
| クローンID | SAA0024 |
| テスト済みアプリケーション | WB, FCM |
| ターゲット | Human epididymis-specific protein 5, CDW52, CAMPATH-1 antigen, Cambridge pathology 1 antigen, He5, HE5, Epididymal secretory protein E5, CD52, CDw52 |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P31358 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 背景 | CAMPATH-1 antigen (CD52) is a ~6 kDa protein. May play a role in carrying and orienting carbohydrate, as well as having a more specific role. |
| 注意事項 | For research use only. |
Flow-cytometry using anti-human CD52 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD52 antibody monoclonal antibody (Catalog # HB894207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD52 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD52 monoclonal antibody (Catalog: HB894207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-CD52 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD52 (Catalog No: HB894011)
Lane 2: negative control
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