Anti-Human L-Selectin/CD62L Recombinant Antibody (Dreg-55) (HB644107)

Name: Anti-Human L-Selectin/CD62L Recombinant Antibody (Dreg-55)
Brand: abinScience
SKU: HB644107
Price: 163 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB644107
Reactivité des espèces	Human
Applications	FCM, Neutralization
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG4
ID de clone	Dreg-55
Applications testées	FCM
Cible	CD62 antigen-like family member L, Leukocyte adhesion molecule 1, CD62L, LYAM1, TQ1, SELL, Leukocyte-endothelial cell adhesion molecule 1, L-selectin, LAM-1, gp90-MEL, LNHR, LECAM1, Lymph node homing receptor, Leukocyte surface antigen Leu-8
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P14151
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	L-selectin (CD62L/SELL) is a ~42 kDa protein. Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. 1. Bernimoulin, MP. et al. (2003) The Journal of biological chemistry 278, 37-47. PMID: 12403782 2. Mehta-D'souza, P. et al. (2017) The Journal of biological chemistry 292, 2510-2518. PMID: 28011641 3. Wedepohl, S. et al. (2017) Chembiochem : a European journal of chemical biology 18, 1338-1345. PMID: 28489325
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD62L/SELL Antibody (Dreg-55).
Flow Cytometry

Flow-cytometry using anti-human SELL antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human SELL antibody monoclonal antibody (Catalog # HB644107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.