Anti-Human L-Selectin/CD62L Recombinant Antibody (Dreg-55) (HB644107)

Name: Anti-Human L-Selectin/CD62L Recombinant Antibody (Dreg-55)
Brand: abinScience
SKU: HB644107
Price: 163 USD
Availability: InStock

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Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер	HB644107
Реактивность видов	Human
Применения	FCM, Neutralization
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG4
ID клона	Dreg-55
Проверенные приложения	FCM
Цель	CD62 antigen-like family member L, Leukocyte adhesion molecule 1, CD62L, LYAM1, TQ1, SELL, Leukocyte-endothelial cell adhesion molecule 1, L-selectin, LAM-1, gp90-MEL, LNHR, LECAM1, Lymph node homing receptor, Leukocyte surface antigen Leu-8
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P14151
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	L-selectin (CD62L/SELL) is a ~42 kDa protein. Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. 1. Bernimoulin, MP. et al. (2003) The Journal of biological chemistry 278, 37-47. PMID: 12403782 2. Mehta-D'souza, P. et al. (2017) The Journal of biological chemistry 292, 2510-2518. PMID: 28011641 3. Wedepohl, S. et al. (2017) Chembiochem : a European journal of chemical biology 18, 1338-1345. PMID: 28489325
Примечание	For research use only.

Изображения

Bioactivity

SEC-HPLC detection for Anti-Human CD62L/SELL Antibody (Dreg-55).
Flow Cytometry

Flow-cytometry using anti-human SELL antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human SELL antibody monoclonal antibody (Catalog # HB644107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.