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| カタログ番号 | HW717217 |
|---|---|
| 種反応性 | Human |
| アプリケーション | FCM |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1, kappa |
| クローンID | 1F6 |
| 抱合 | FITC |
| テスト済みアプリケーション | FCM |
| ターゲット | CD27 ligand, CD70, TNFSF7, CD27LG, CD27L, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, CD27-L |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 関連製品 | Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC |
| アクセッション番号 | P32970 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 背景 | CD70 antigen is a ~21 kDa protein. Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Abolhassani, H. et al. (2017) The Journal of experimental medicine 214, 91-106. PMID: 28011864 3. Goodwin, RG. et al. (1993) Cell 73, 447-56. PMID: 8387892 |
| 注意事項 | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |
Flow-cytometry using FITC anti-human CD70 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD70 monoclonal antibody (Catalog HW717217, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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