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Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6) (HW717207)

Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6)
Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6)
Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6)
Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6)
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  • Обзор

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  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерHW717207
Реактивность видовHuman
ПримененияFCM
Хозяинский видHuman
КлоональностьMonoclonal
ИзотипIgG1, kappa
ID клона1F6
Проверенные приложения FCM
Цель CD27 ligand, CD70, TNFSF7, CD27LG, CD27L, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, CD27-L
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P32970
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон

CD70 antigen is a ~21 kDa protein. Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells.

1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863
2. Abolhassani, H. et al. (2017) The Journal of experimental medicine 214, 91-106. PMID: 28011864
3. Goodwin, RG. et al. (1993) Cell 73, 447-56. PMID: 8387892
Примечание For research use only.
Изображения
  • Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD70 Antibody (1F6).

  • Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6)

    Flow CytoMetry

    Flow-cytometry using anti-human CD70 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD70 monoclonal antibody (Catalog: HW717207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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