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Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE (HW717227)

Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE
Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE
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  • 100T
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Vista general
Número de catálogoHW717227
Reactividad de especiesHuman
AplicacionesFCM
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clon1F6
ConjugaciónPE
Aplicaciones probadas FCM
Objetivo CD27 ligand, CD70, TNFSF7, CD27LG, CD27L, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, CD27-L
Nivel de endotoxinas Please contact with the lab for this information.
Productos asociados Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), PE
Número de acceso P32970
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

CD70 antigen is a ~21 kDa protein. Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells.

1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863
2. Abolhassani, H. et al. (2017) The Journal of experimental medicine 214, 91-106. PMID: 28011864
3. Goodwin, RG. et al. (1993) Cell 73, 447-56. PMID: 8387892
Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE

    Flow cytometry

    Flow-cytometry using PE anti-human CD70 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD70 monoclonal antibody (Catalog HW717227, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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