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Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE (HW717227)

Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE
Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE
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Spécification :
  • 50T
  • 100T
Nombre :
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Aperçu
Numéro de catalogueHW717227
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de clone1F6
ConjugaisonPE
Applications testées FCM
Cible CD27 ligand, CD70, TNFSF7, CD27LG, CD27L, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, CD27-L
Niveau d'endotoxines Please contact with the lab for this information.
Produits associés Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), PE
Numéro d'accès P32970
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

CD70 antigen is a ~21 kDa protein. Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells.

1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863
2. Abolhassani, H. et al. (2017) The Journal of experimental medicine 214, 91-106. PMID: 28011864
3. Goodwin, RG. et al. (1993) Cell 73, 447-56. PMID: 8387892
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE

    Flow cytometry

    Flow-cytometry using PE anti-human CD70 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD70 monoclonal antibody (Catalog HW717227, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Volume
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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