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Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE (HW717227)

Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE
Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE
Цена(USD):
Спецификация:
  • 50T
  • 100T
Количество:
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Обзор
Каталожный номерHW717227
Реактивность видовHuman
ПримененияFCM
Хозяинский видHuman
КлоональностьMonoclonal
ИзотипIgG1, kappa
ID клона1F6
КонъюгацияPE
Проверенные приложения FCM
Цель CD27 ligand, CD70, TNFSF7, CD27LG, CD27L, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, CD27-L
Уровень эндотоксинов Please contact with the lab for this information.
Связанные продукты Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), PE
Номер доступа P32970
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон

CD70 antigen is a ~21 kDa protein. Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells.

1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863
2. Abolhassani, H. et al. (2017) The Journal of experimental medicine 214, 91-106. PMID: 28011864
3. Goodwin, RG. et al. (1993) Cell 73, 447-56. PMID: 8387892
Примечание For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Изображения
  • Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6), PE

    Flow cytometry

    Flow-cytometry using PE anti-human CD70 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD70 monoclonal antibody (Catalog HW717227, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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