Anti-Human Transferrin R/TfR Reference Recombinant Antibody (JR-141) (HY036107)

Name: Anti-Human Transferrin R/TfR Reference Recombinant Antibody (JR-141)
Brand: abinScience
SKU: HY036107
Price: 196 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HY036107
種反応性	Human
アプリケーション	ELISA, FCM
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	JR-141
テスト済みアプリケーション	FCM
ターゲット	CD71, T9, TFRC, TR, TfR, TfR1, Transferrin receptor 1, Transferrin receptor protein 1, Transferrin receptor protein 1, serum form, Trfr, p90, sTfR
エンドトキシンレベル	< 10 EU/mg
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P02786
形態	Liquid
保存バッファー	0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景	Transferrin receptor protein 1 (CD71/TFRC) is a ~84 kDa protein. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. 1. Senyilmaz, D. et al. (2015) Nature 525, 124-8. PMID: 26214738 2. Jabara, HH. et al. (2016) Nature genetics 48, 74-8. PMID: 26642240
注意事項	For research use only.

イメージ

Flow-cytometry

Flow-cytometry using anti-human CD71 antibody.CD71 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 antibody monoclonal antibody (Catalog # HY036107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD71 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 antibody monoclonal antibody (Catalog # HY036107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD71/TFRC Antibody (JR-141).
Flow CytoMetry

Flow-cytometry using anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 monoclonal antibody (Catalog: HY036107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.