Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), APC (HB209337)

Name: Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), APC
Brand: abinScience
SKU: HB209337
Price: 214 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB209337
Reactivité des espèces	Human
Applications	FCM
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	SAA0034
Conjugaison	APC
Applications testées	FCM
Cible	5'-NT, 5'-deoxynucleotidase, 5'-nucleotidase, CD73, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, NT5, NT5E, NTE, Thymidylate 5'-phosphatase
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Produits associés	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Numéro d'accès	P21589
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. 1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152 2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138 3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347 4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587 5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using APC anti-human CD73 antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD73 monoclonal antibody (Catalog: HB209337, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

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