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Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), APC (HB209337)

Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), APC
Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), APC
Цена(USD):
Спецификация:
  • 50T
  • 100T
Количество:
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  • Обзор

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  • Даташит

  • SDS

Обзор
Каталожный номерHB209337
Реактивность видовHuman
ПримененияFCM
Хозяинский видMouse
КлоональностьMonoclonal
ИзотипIgG1, kappa
ID клонаSAA0034
КонъюгацияAPC
Проверенные приложения FCM
Цель 5'-NT, 5'-deoxynucleotidase, 5'-nucleotidase, CD73, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, NT5, NT5E, NTE, Thymidylate 5'-phosphatase
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Связанные продукты Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Номер доступа P21589
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон

5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.

1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152
2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138
3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347
4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587
5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084
Примечание For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Изображения
  • Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human CD73 antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD73 monoclonal antibody (Catalog: HB209337, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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