Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), PE (HB209327)

Name: Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), PE
Brand: abinScience
SKU: HB209327
Price: 214 USD
Availability: InStock

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Vista general

Número de catálogo	HB209327
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG1, kappa
ID de clon	SAA0034
Conjugación	PE
Aplicaciones probadas	FCM
Objetivo	5'-NT, 5'-deoxynucleotidase, 5'-nucleotidase, CD73, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, NT5, NT5E, NTE, Thymidylate 5'-phosphatase
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Productos asociados	Mouse IgG1 kappa Recombinant Isotype Control Antibody (MOPC-21), PE
Número de acceso	P21589
Forma	Liquid
Buffer de almacenamiento	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo	5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. 1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152 2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138 3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347 4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587 5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084
Nota	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using PE anti-human CD73 antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD73 monoclonal antibody (Catalog: HB209327, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet