Anti-Human B7-2/CD86 Recombinant Antibody (3D1) (HW776023)

Name: Anti-Human B7-2/CD86 Recombinant Antibody (3D1)
Brand: abinScience
SKU: HW776023
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HW776023

Reactividad de especies

Human

Aplicaciones

ELISA, FCM, IP

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

3D1

Aplicaciones probadas

WB, FCM

Objetivo

T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD86, CD28LG2, B7-2

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P42081, K7GMU1

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

T-lymphocyte activation antigen CD86 (B7-2) is a ~37 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.

1. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291

2. Freeman, GJ. et al. (1993) Science (New York, N.Y.) 262, 909-11. PMID: 7694363

3. Lanier, LL. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 97-105. PMID: 7527824

4. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501

Nota

For research use only

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Imágenes

Western blot

Western blot analysis was performed using anti-CD86/B7-2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD86/B7-2 (Catalog No: HW776011)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human CD86 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 monoclonal antibody (Catalog HW776023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias

Datasheet

Anti-Human B7-2/CD86 Recombinant Antibody (3D1).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo