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Anti-Human CEL Polyclonal Antibody (HB688014)

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Aperçu
Numéro de catalogueHB688014
Description
Anti-Human CEL Polyclonal Antibody (HB688014) is a rabbit polyclonal antibody detecting CEL in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CEL (Asp117-Glu361).
Cible Carboxyl ester lipase, Bucelipase, Sterol esterase, Bile salt-activated lipase, BAL, Bile salt-stimulated lipase, Cholesterol esterase, Pancreatic lysophospholipase, CEL, BSSL
Purification Purified by antigen affinity column.
Numéro d'accès P19835
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Bile salt-activated lipase (CEL) is a ~79 kDa protein. Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs). Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs. Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins.

1. Tanaka, H. et al. (1999) Journal of biochemistry 125, 883-90. PMID: 10220579
2. Kolar, MJ. et al. (2016) Biochemistry 55, 4636-41. PMID: 27509211
3. Xiao, X. et al. (2016) The Journal of biological chemistry 291, 23224-23236. PMID: 27650499
4. Hui, DY. et al. (1993) The Biochemical journal 291 ( Pt 1), 65-9. PMID: 8471055
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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