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Anti-Human CEL Polyclonal Antibody (HB688014)

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概要
カタログ番号HB688014
説明
Anti-Human CEL Polyclonal Antibody (HB688014) is a rabbit polyclonal antibody detecting CEL in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Human
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human CEL (Asp117-Glu361).
ターゲット Carboxyl ester lipase, Bucelipase, Sterol esterase, Bile salt-activated lipase, BAL, Bile salt-stimulated lipase, Cholesterol esterase, Pancreatic lysophospholipase, CEL, BSSL
精製 Purified by antigen affinity column.
アクセッション番号 P19835
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Bile salt-activated lipase (CEL) is a ~79 kDa protein. Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs). Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs. Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins.

1. Tanaka, H. et al. (1999) Journal of biochemistry 125, 883-90. PMID: 10220579
2. Kolar, MJ. et al. (2016) Biochemistry 55, 4636-41. PMID: 27509211
3. Xiao, X. et al. (2016) The Journal of biological chemistry 291, 23224-23236. PMID: 27650499
4. Hui, DY. et al. (1993) The Biochemical journal 291 ( Pt 1), 65-9. PMID: 8471055
注意事項 For research use only.
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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