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Обзор
Каталожный номерHB693014
Описание
Anti-Human CES1 Polyclonal Antibody (HB693014) is a rabbit polyclonal antibody detecting CES1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human CES1 (Gly18-Leu299).
Цель Serine esterase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, Liver carboxylesterase 1, REH, Methylumbelliferyl-acetate deacetylase 1, HMSE, ACAT, hCE-1, Acyl-coenzyme A:cholesterol acyltransferase, CE-1, Brain carboxylesterase hBr1, CEH, SES1, CES2, Cholesteryl ester hydrolase, Triacylglycerol hydrolase, Cocaine carboxylesterase, TGH, Carboxylesterase 1, CES1, Egasyn
Очистка Purified by antigen affinity column.
Номер доступа P23141
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Liver carboxylesterase 1 (CES1) is a ~62 kDa protein. Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.

1. Crow, JA. et al. (2008) Biochimica et biophysica acta 1781, 643-54. PMID: 18762277
2. Brzezinski, MR. et al. (1994) Biochemical pharmacology 48, 1747-55. PMID: 7980644
3. Pindel, EV. et al. (1997) The Journal of biological chemistry 272, 14769-75. PMID: 9169443
4. Schindler, R. et al. (1998) European journal of biochemistry 251, 863-73. PMID: 9490062
5. Xie, S. et al. (2010) Chemical research in toxicology 23, 1890-904. PMID: 21049984
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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