Anti-Human CFH Recombinant Nanobody (SAA2038) (HY505023)

Name: Anti-Human CFH Recombinant Nanobody (SAA2038)
Brand: abinScience
SKU: HY505023
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY505023

Descripción

Anti-Human CFH Nanobody (SAA2038) [SAA2038] (HY505023) is a alpaca monoclonal antibody detecting CFH in ELISA. Suitable for Human.

Reactividad de especies

Human

Aplicaciones

ELISA

Especie huésped

Alpaca

Clonalidad

Monoclonal

Isotipo

VHH-8His-Cys-tag

ID de clon

SAA2038

Objetivo

Complement factor H, CFH, H factor 1, HF1, HF, HF2

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Purified by Nickel column.

Número de acceso

P08603

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Complement factor H (CFH) is a ~139 kDa protein. Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors.

1. Kajander, T. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 2897-902. PMID: 21285368

2. Morgan, HP. et al. (2011) Nature structural & molecular biology 18, 463-70. PMID: 21317894

3. Blaum, BS. et al. (2015) Nature chemical biology 11, 77-82. PMID: 25402769

4. Wu, J. et al. (2009) Nature immunology 10, 728-33. PMID: 19503104

5. Kennedy, AT. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 196, 1239-48. PMID: 26700768

6. Hocking, HG. et al. (2008) The Journal of biological chemistry 283, 9475-87. PMID: 18252712

7. Simon, N. et al. (2013) Cell host & microbe 13, 29-41. PMID: 23332154

8. Xue, X. et al. (2017) Nature structural & molecular biology 24, 643-651. PMID: 28671664

9. Losse, J. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 184, 912-21. PMID: 20008295

10. DiScipio, RG. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 4057-66. PMID: 9558116

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human CFH Recombinant Nanobody (SAA2038)
Bioactivity

Detects recombinant CFH (Catalog No: HY505012) in indirect ELISA.
Immunofluorescence

Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Secreted.