Anti-Human CFH Recombinant Nanobody (SAA2038) (HY505023)

Name: Anti-Human CFH Recombinant Nanobody (SAA2038)
Brand: abinScience
SKU: HY505023
Price: 126 USD
Availability: InStock

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20ug
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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY505023

説明

Anti-Human CFH Nanobody (SAA2038) [SAA2038] (HY505023) is a alpaca monoclonal antibody detecting CFH in ELISA. Suitable for Human.

種反応性

Human

アプリケーション

ELISA

宿主種

Alpaca

クローン性

Monoclonal

アイソタイプ

VHH-8His-Cys-tag

クローンID

SAA2038

ターゲット

Complement factor H, CFH, H factor 1, HF1, HF, HF2

エンドトキシンレベル

Please contact with the lab for this information.

純度

>95% as determined by SDS-PAGE.

精製

Purified by Nickel column.

アクセッション番号

P08603

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Complement factor H (CFH) is a ~139 kDa protein. Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors.

1. Kajander, T. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 2897-902. PMID: 21285368

2. Morgan, HP. et al. (2011) Nature structural & molecular biology 18, 463-70. PMID: 21317894

3. Blaum, BS. et al. (2015) Nature chemical biology 11, 77-82. PMID: 25402769

4. Wu, J. et al. (2009) Nature immunology 10, 728-33. PMID: 19503104

5. Kennedy, AT. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 196, 1239-48. PMID: 26700768

6. Hocking, HG. et al. (2008) The Journal of biological chemistry 283, 9475-87. PMID: 18252712

7. Simon, N. et al. (2013) Cell host & microbe 13, 29-41. PMID: 23332154

8. Xue, X. et al. (2017) Nature structural & molecular biology 24, 643-651. PMID: 28671664

9. Losse, J. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 184, 912-21. PMID: 20008295

10. DiScipio, RG. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 4057-66. PMID: 9558116

注意事項

For research use only.

イメージ

SDS-PAGE

SDS-PAGE for Anti-Human CFH Recombinant Nanobody (SAA2038)
Bioactivity

Detects recombinant CFH (Catalog No: HY505012) in indirect ELISA.
Immunofluorescence

Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Secreted.