Anti-Human CHEK1 Polyclonal Antibody (HA503014)

Name: Anti-Human CHEK1 Polyclonal Antibody
Brand: abinScience
SKU: HA503014
Price: 96 USD
Availability: InStock

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Número de catálogo

HA503014

Descripción

Anti-Human CHEK1 Polyclonal Antibody (HA503014) is a rabbit polyclonal antibody detecting CHEK1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Gallus gallus, Mus musculus, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CHEK1 (Met1-Asn266).

Objetivo

CHEK1

Purificación

Purified by antigen affinity column.

Número de acceso

O14757

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Nombres alternativos

Serine/threonine-protein kinase Chk1, 2.7.11.1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1

Fondo

Serine/threonine-protein kinase Chk1 (CHEK1) is a ~54 kDa protein. Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C.

1. Feijoo, C. et al. (2001) The Journal of cell biology 154, 913-23. PMID: 11535615

2. Zhao, H. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 14795-800. PMID: 12399544

3. Heffernan, TP. et al. (2002) Molecular and cellular biology 22, 8552-61. PMID: 12446774

4. Chen, MS. et al. (2003) Molecular and cellular biology 23, 7488-97. PMID: 14559997

5. Pichierri, P. et al. (2004) The EMBO journal 23, 1178-87. PMID: 14988723

6. Krämer, A. et al. (2004) Nature cell biology 6, 884-91. PMID: 15311285

7. Huang, X. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1065-70. PMID: 15650047

8. Sørensen, CS. et al. (2005) Nature cell biology 7, 195-201. PMID: 15665856

9. Yu, X. et al. (2020) Journal of hematology & oncology 13, 40. PMID: 32357935

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-CHEK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate

Referencias

Datasheet

Anti-Human CHEK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human CHEK1 Polyclonal Antibody (HA503014)

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Imágenes

Referencias

Datasheet

SDS