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Anti-Human CIAO2B Polyclonal Antibody (HP061014)

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Aperçu
Numéro de catalogueHP061014
Description
Anti-Human CIAO2B Polyclonal Antibody (HP061014) is a rabbit polyclonal antibody detecting CIAO2B in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CIAO2B (Thr29-Ser163).
Cible Mitotic spindle-associated MMXD complex subunit MIP18, FAM96B, Protein FAM96B, MSS19-interacting protein of 18 kDa, CIAB, CIAO2B, MIP18, Cytosolic iron-sulfur assembly component 2B
Purification Purified by antigen affinity column.
Numéro d'accès Q9Y3D0
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cytosolic iron-sulfur assembly component 2B (CIAO2B) is a ~17 kDa protein. Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As a CIA complex component and in collaboration with CIAO1 and MMS19, binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. Together with MMS19, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis.

1. Gari, K. et al. (2012) Science (New York, N.Y.) 337, 243-5. PMID: 22678361
2. Stehling, O. et al. (2012) Science (New York, N.Y.) 337, 195-9. PMID: 22678362
3. Stehling, O. et al. (2013) Cell metabolism 18, 187-98. PMID: 23891004
4. Ben-Shimon, L. et al. (2018) Journal of cell science 131. PMID: 29848660
5. Ito, S. et al. (2010) Molecular cell 39, 632-40. PMID: 20797633
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human CIAO2B Polyclonal Antibody

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