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Anti-Human CNOT7 Polyclonal Antibody (HA970014)

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Aperçu
Numéro de catalogueHA970014
Description
Anti-Human CNOT7 Polyclonal Antibody (HA970014) is a rabbit polyclonal antibody detecting CNOT7 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CNOT7 (Arg11-Ser285).
Cible CNOT7, BTG1-binding factor 1, CAF-1, CAF1, Caf1a, CCR4-NOT transcription complex subunit 7, CCR4-associated factor 1
Purification Purified by antigen affinity column.
Numéro d'accès Q9UIV1
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

CCR4-NOT transcription complex subunit 7 (CNOT7) is a ~32 kDa protein. Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex also seems to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression.

1. Horiuchi, M. et al. (2009) The Journal of biological chemistry 284, 13244-55. PMID: 19276069
2. Cooke, A. et al. (2010) The Journal of biological chemistry 285, 28506-13. PMID: 20634287
3. Kim, TH. et al. (2019) Science (New York, N.Y.) 365, 825-829. PMID: 31439799
4. Aslam, A. et al. (2009) Molecular biology of the cell 20, 3840-50. PMID: 19605561
5. Piao, X. et al. (2010) Molecular and cellular biology 30, 1486-94. PMID: 20065043
6. Doidge, R. et al. (2012) PloS one 7, e51331. PMID: 23236473
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human CNOT7 Polyclonal Antibody

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