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| カタログ番号 | HS787014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human CPSF6 (Lys79-Ser181). | ||||||||
| ターゲット | Pre-mRNA cleavage factor Im 68 kDa subunit, CFIM68, Protein HPBRII-4/7, Cleavage and polyadenylation specificity factor subunit 6, CPSF6, CFIm68, CPSF 68 kDa subunit, Cleavage and polyadenylation specificity factor 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q16630 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) is a ~59 kDa protein. Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. 1. Brown, KM. et al. (2003) Molecular cell 12, 1467-76. PMID: 14690600 2. Zhu, Y. et al. (2018) Molecular cell 69, 62-74.e4. PMID: 29276085 3. Rüegsegger, U. et al. (1996) The Journal of biological chemistry 271, 6107-13. PMID: 8626397 4. Rüegsegger, U. et al. (1998) Molecular cell 1, 243-53. PMID: 9659921 5. Gruber, AR. et al. (2012) RNA biology 9, 1405-12. PMID: 23187700 6. Kim, S. et al. (2010) Genes to cells : devoted to molecular & cellular mechanisms 15, 1003-13. PMID: 20695905 | ||||||||
| 注意事項 | For research use only. |
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