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Anti-Human CRCP Polyclonal Antibody (HF859014)

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Aperçu
Numéro de catalogueHF859014
Description
Anti-Human CRCP Polyclonal Antibody (HF859014) is a rabbit polyclonal antibody detecting CRCP in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CRCP (Ser12-Ala148).
Cible CGRPRCP, HsC17, Calcitonin gene-related peptide-receptor component protein, CGRP-RCP, CRCP, RNA polymerase III subunit C9, DNA-directed RNA polymerase III subunit RPC9, CGRP-receptor component protein
Purification Purified by antigen affinity column.
Numéro d'accès O75575
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

DNA-directed RNA polymerase III subunit RPC9 (CRCP) is a ~16 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. With POLR3H/RPC8 forms a mobile stalk that protrudes from Pol III core and functions primarily in transcription initiation. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response.

1. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673
2. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764
3. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218
4. Wang, Q. et al. (2021) Nature structural & molecular biology 28, 220-227. PMID: 33558766
5. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254
6. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370
Note For research use only.
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CRCP Polyclonal Antibody

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