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Anti-Human M-CSF Recombinant Antibody (SAA0417), APC (HY515137)

Anti-Human M-CSF Recombinant Antibody (SAA0417), APC
Anti-Human M-CSF Recombinant Antibody (SAA0417), APC
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Aperçu
Numéro de catalogueHY515137
Reactivité des espècesHuman
ApplicationsELISA, FCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneSAA0417
ConjugaisonAPC
Cible CSF1, Macrophage colony-stimulating factor 1, CSF-1, Lanimostim, M-CSF, MCSF
Niveau d'endotoxines Please contact with the lab for this information.
Produits associés Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), APC
Numéro d'accès P09603
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

Macrophage colony-stimulating factor 1 (CSF1/M-CSF) is a ~60 kDa protein. Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration.

Reférences 1. Jia S, et al. Tuning the Color Palette of Fluorescent Copper Sensors through Systematic Heteroatom Substitution at Rhodol Cores. ACS Chem Biol. 2018;13(7):1844-1852. [HY515137]
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human M-CSF Recombinant Antibody (SAA0417), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CSF1 monoclonal antibody (Catalog: HY515137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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