Anti-Human CTNNB1 Recombinant Nanobody (SAA1410) (HW518033)

Name: Anti-Human CTNNB1 Recombinant Nanobody (SAA1410)
Brand: abinScience
SKU: HW518033
Price: 126 USD
Availability: InStock

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1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HW518033

Description

Anti-Human CTNNB1 Nanobody (SAA1410) [SAA1410] (HW518033) is a alpaca monoclonal antibody detecting Human CTNNB1 in ELISA. Suitable for Human.

Reactivité des espèces

Human

Applications

ELISA

Espèce hôte

Alpaca

Clonalité

Monoclonal

Isotype

VHH-His

ID de clone

SAA1410

Cible

BC2 peptide-tag, BC2e-tag, PDRKAAVSHWQQ

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Numéro d'accès

P35222

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Catenin beta-1 (CTNNB1) is a ~85 kDa protein. Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Also acts as a coactivator for other transcription factors, such as NR5A2. Promotes epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via driving transcription of CTNNB1/TCF-target genes.

1. Lillehoj, EP. et al. (2007) Biochimica et biophysica acta 1773, 1028-38. PMID: 17524503

2. Weiske, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20344-9. PMID: 18077326

3. Bahmanyar, S. et al. (2008) Genes & development 22, 91-105. PMID: 18086858

4. Li, H. et al. (2009) The Journal of biological chemistry 284, 2012-22. PMID: 18957423

5. Fiset, A. et al. (2011) Cellular signalling 23, 911-9. PMID: 21262353

6. Satow, R. et al. (2012) Gastroenterology 142, 572-81. PMID: 22155184

7. Genovese, G. et al. (2012) Cell cycle (Georgetown, Tex.) 11, 2206-15. PMID: 22647378

8. Yu, Y. et al. (2012) EMBO reports 13, 750-8. PMID: 22699938

10. Park, J. et al. (2018) Stem cell reports 11, 115-127. PMID: 29910125

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CTNNB1 Nanobody (SAA1410)

Reférences

Fiche technique

Anti-Human CTNNB1 Recombinant Nanobody (SAA1410).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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