Anti-Human CUL4B Polyclonal Antibody (HC617014)

Name: Anti-Human CUL4B Polyclonal Antibody
Brand: abinScience
SKU: HC617014
Price: 168 USD
Availability: InStock

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Referencias
Datasheet
SDS

Vista general

Número de catálogo

HC617014

Descripción

Anti-Human CUL4B Polyclonal Antibody (HC617014) is a rabbit polyclonal antibody detecting CUL4B in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CUL4B (Gly728-Ala913).

Objetivo

Cullin-4B, CUL-4B, CUL4B, KIAA0695

Purificación

Purified by antigen affinity column.

Número de acceso

Q13620

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Cullin-4B (CUL4B) is a ~103 kDa protein. Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication.

1. Higa, LA. et al. (2003) Nature cell biology 5, 1008-15. PMID: 14578910

2. Higa, LA. et al. (2006) Cell cycle (Georgetown, Tex.) 5, 71-7. PMID: 16322693

3. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110

4. Guerrero-Santoro, J. et al. (2008) Cancer research 68, 5014-22. PMID: 18593899

5. Fischer, ES. et al. (2011) Cell 147, 1024-39. PMID: 22118460

6. Koren, I. et al. (2018) Cell 173, 1622-1635.e14. PMID: 29779948

7. Chen, SH. et al. (2018) The EMBO journal 37. PMID: 30166453

8. Maiani, E. et al. (2021) Nature 592, 799-803. PMID: 33854232

9. Chaikovsky, AC. et al. (2021) Nature 592, 794-798. PMID: 33854239

10. Sjögren, B. et al. (2015) PloS one 10, e0123581. PMID: 25970626

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human CUL4B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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