Anti-Human CUL4B Polyclonal Antibody (HC617014)

Name: Anti-Human CUL4B Polyclonal Antibody
Brand: abinScience
SKU: HC617014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HC617014

Description

Anti-Human CUL4B Polyclonal Antibody (HC617014) is a rabbit polyclonal antibody detecting CUL4B in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human CUL4B (Gly728-Ala913).

Cible

Cullin-4B, CUL-4B, CUL4B, KIAA0695

Purification

Purified by antigen affinity column.

Numéro d'accès

Q13620

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Cullin-4B (CUL4B) is a ~103 kDa protein. Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication.

1. Higa, LA. et al. (2003) Nature cell biology 5, 1008-15. PMID: 14578910

2. Higa, LA. et al. (2006) Cell cycle (Georgetown, Tex.) 5, 71-7. PMID: 16322693

3. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110

4. Guerrero-Santoro, J. et al. (2008) Cancer research 68, 5014-22. PMID: 18593899

5. Fischer, ES. et al. (2011) Cell 147, 1024-39. PMID: 22118460

6. Koren, I. et al. (2018) Cell 173, 1622-1635.e14. PMID: 29779948

7. Chen, SH. et al. (2018) The EMBO journal 37. PMID: 30166453

8. Maiani, E. et al. (2021) Nature 592, 799-803. PMID: 33854232

9. Chaikovsky, AC. et al. (2021) Nature 592, 794-798. PMID: 33854239

10. Sjögren, B. et al. (2015) PloS one 10, e0123581. PMID: 25970626

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human CUL4B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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