Anti-Human CUL4B Polyclonal Antibody (HC617014)

Name: Anti-Human CUL4B Polyclonal Antibody
Brand: abinScience
SKU: HC617014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

HC617014

Описание

Anti-Human CUL4B Polyclonal Antibody (HC617014) is a rabbit polyclonal antibody detecting CUL4B in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human CUL4B (Gly728-Ala913).

Цель

Cullin-4B, CUL-4B, CUL4B, KIAA0695

Очистка

Purified by antigen affinity column.

Номер доступа

Q13620

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Cullin-4B (CUL4B) is a ~103 kDa protein. Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication.

1. Higa, LA. et al. (2003) Nature cell biology 5, 1008-15. PMID: 14578910

2. Higa, LA. et al. (2006) Cell cycle (Georgetown, Tex.) 5, 71-7. PMID: 16322693

3. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110

4. Guerrero-Santoro, J. et al. (2008) Cancer research 68, 5014-22. PMID: 18593899

5. Fischer, ES. et al. (2011) Cell 147, 1024-39. PMID: 22118460

6. Koren, I. et al. (2018) Cell 173, 1622-1635.e14. PMID: 29779948

7. Chen, SH. et al. (2018) The EMBO journal 37. PMID: 30166453

8. Maiani, E. et al. (2021) Nature 592, 799-803. PMID: 33854232

9. Chaikovsky, AC. et al. (2021) Nature 592, 794-798. PMID: 33854239

10. Sjögren, B. et al. (2015) PloS one 10, e0123581. PMID: 25970626

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human CUL4B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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