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Anti-Human CUL7 Polyclonal Antibody (HD226014)

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Vista general
Número de catálogoHD226014
Descripción
Anti-Human CUL7 Polyclonal Antibody (HD226014) is a rabbit polyclonal antibody detecting CUL7. Suitable for Human.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CUL7 (Met1-Ser317).
Objetivo CUL-7, CUL7, Cullin-7, KIAA0076
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q14999
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Cullin-7 (CUL7) is a ~191 kDa protein. Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. The Cul7-RING(FBXW8) complex alone lacks ubiquitination activity and does not promote polyubiquitination and proteasomal degradation of p53/TP53. However it mediates recruitment of p53/TP53 for ubiquitination by neddylated CUL1-RBX1.

1. Dias, DC. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 16601-6. PMID: 12481031
2. Arai, T. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 9855-60. PMID: 12904573
3. Litterman, N. et al. (2011) PLoS biology 9, e1001060. PMID: 21572988
4. Hanson, D. et al. (2011) American journal of human genetics 89, 148-53. PMID: 21737058
5. Yan, J. et al. (2014) Molecular cell 54, 791-804. PMID: 24793695
6. Hopf, LVM. et al. (2022) Nature structural & molecular biology 29, 854-862. PMID: 35982156
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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