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Anti-Human CYP11A1 Polyclonal Antibody (HY252014)

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Anti-Human CYP11A1 Polyclonal Antibody
Anti-Human CYP11A1 Polyclonal Antibody
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Vista general
Número de catálogoHY252014
Descripción
Anti-Human CYP11A1 Polyclonal Antibody (HY252014) is a rabbit polyclonal antibody detecting CYP11A1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CYP11A1 (Met1-Gly300).
Objetivo Cholesterol side-chain cleavage enzyme, mitochondrial, CYPXIA1, CYP11A, Cholesterol desmolase, Cytochrome P450 11A1, CYP11A1, Cytochrome P450(scc)
Purificación Purified by antigen affinity column.
Número de acceso P05108
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1) is a ~60 kDa protein. A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).

1. Strushkevich, N. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 10139-43. PMID: 21636783
Nota For research use only.
Imágenes
  • Anti-Human CYP11A1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CYP11A1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse ovary lysate
    Lane 2: Rat ovary lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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