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Anti-Human CYP11A1 Polyclonal Antibody (HY252014)

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Anti-Human CYP11A1 Polyclonal Antibody
Anti-Human CYP11A1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHY252014
Description
Anti-Human CYP11A1 Polyclonal Antibody (HY252014) is a rabbit polyclonal antibody detecting CYP11A1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CYP11A1 (Met1-Gly300).
Cible Cholesterol side-chain cleavage enzyme, mitochondrial, CYPXIA1, CYP11A, Cholesterol desmolase, Cytochrome P450 11A1, CYP11A1, Cytochrome P450(scc)
Purification Purified by antigen affinity column.
Numéro d'accès P05108
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1) is a ~60 kDa protein. A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).

1. Strushkevich, N. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 10139-43. PMID: 21636783
Note For research use only.
Images
  • Anti-Human CYP11A1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CYP11A1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse ovary lysate
    Lane 2: Rat ovary lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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