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Anti-Human CYP1A2 Polyclonal Antibody (HY226014)

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Aperçu
Numéro de catalogueHY226014
Description
Anti-Human CYP1A2 Polyclonal Antibody (HY226014) is a rabbit polyclonal antibody detecting CYP1A2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CYP1A2 (Ala2-Ser231).
Cible Hydroperoxy icosatetraenoate dehydratase, Cytochrome P450-P3, Cytochrome P450 4, CYP1A2, Cholesterol 25-hydroxylase, CYPIA2, Cytochrome P(3)450, Cytochrome P450 1A2
Purification Purified by antigen affinity column.
Numéro d'accès P05177
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cytochrome P450 1A2 (CYP1A2) is a ~58 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis.

1. Chen, H. et al. (2000) Drug metabolism and disposition: the biological fate of chemicals 28, 315-22. PMID: 10681376
2. Badawi, AF. et al. (2001) Metabolism: clinical and experimental 50, 1001-3. PMID: 11555828
3. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317
4. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576
5. Bylund, J. et al. (1998) The Journal of pharmacology and experimental therapeutics 284, 51-60. PMID: 9435160
6. Honda, A. et al. (2011) Journal of lipid research 52, 1509-16. PMID: 21576599
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CYP1A2 Polyclonal Antibody

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