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Anti-Human CYP2C9 Polyclonal Antibody (HB549014)

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Vista general
Número de catálogoHB549014
Descripción
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human CYP2C9 (Gly40-Val490).
Objetivo Cytochrome P450 2C9; 1.14.14.1; (R)-limonene 6-monooxygenase; 1.14.14.53; (S)-limonene 6-monooxygenase; 1.14.14.51; (S)-limonene 7-monooxygenase; 1.14.14.52; CYPIIC9; Cholesterol 25-hydroxylase; Cytochrome P-450MP; Cytochrome P450 MP-4; Cytochrome P450 MP-8; Cytochrome P450 PB-1; S-mephenytoin 4-hydroxylase; CYP2C9; CYP2C10
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P11712, P33261
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Cytochrome P450 2C9 (CYP2C9) is a ~55 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis.

1. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317
2. Barbosa-Sicard, E. et al. (2005) Biochemical and biophysical research communications 329, 1275-81. PMID: 15766564
3. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576
4. Honda, A. et al. (2011) Journal of lipid research 52, 1509-16. PMID: 21576599
5. Zeldin, DC. et al. (1995) Archives of biochemistry and biophysics 322, 76-86. PMID: 7574697
6. Bylund, J. et al. (1998) The Journal of pharmacology and experimental therapeutics 284, 51-60. PMID: 9435160
7. Bylund, J. et al. (1998) Analytical biochemistry 265, 55-68. PMID: 9866708
8. Miyazawa, M. et al. (2002) Drug metabolism and disposition: the biological fate of chemicals 30, 602-7. PMID: 11950794
Nota For research use only
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CYP2C9 Polyclonal Antibody

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