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Anti-Human CYP2C9 Polyclonal Antibody (HB549014)

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Aperçu
Numéro de catalogueHB549014
Description
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human CYP2C9 (Gly40-Val490).
Cible Cytochrome P450 2C9; 1.14.14.1; (R)-limonene 6-monooxygenase; 1.14.14.53; (S)-limonene 6-monooxygenase; 1.14.14.51; (S)-limonene 7-monooxygenase; 1.14.14.52; CYPIIC9; Cholesterol 25-hydroxylase; Cytochrome P-450MP; Cytochrome P450 MP-4; Cytochrome P450 MP-8; Cytochrome P450 PB-1; S-mephenytoin 4-hydroxylase; CYP2C9; CYP2C10
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P11712, P33261
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cytochrome P450 2C9 (CYP2C9) is a ~55 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis.

1. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317
2. Barbosa-Sicard, E. et al. (2005) Biochemical and biophysical research communications 329, 1275-81. PMID: 15766564
3. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576
4. Honda, A. et al. (2011) Journal of lipid research 52, 1509-16. PMID: 21576599
5. Zeldin, DC. et al. (1995) Archives of biochemistry and biophysics 322, 76-86. PMID: 7574697
6. Bylund, J. et al. (1998) The Journal of pharmacology and experimental therapeutics 284, 51-60. PMID: 9435160
7. Bylund, J. et al. (1998) Analytical biochemistry 265, 55-68. PMID: 9866708
8. Miyazawa, M. et al. (2002) Drug metabolism and disposition: the biological fate of chemicals 30, 602-7. PMID: 11950794
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CYP2C9 Polyclonal Antibody

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