Anti-Human CYP46A1 Polyclonal Antibody (HV358014)

Name: Anti-Human CYP46A1 Polyclonal Antibody
Brand: abinScience
SKU: HV358014
Price: 168 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD): --

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HV358014

Descripción

Anti-Human CYP46A1 Polyclonal Antibody (HV358014) is a rabbit polyclonal antibody detecting CYP46A1 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CYP46A1 (His45-Cys500).

Objetivo

Cholesterol 24-hydroxylase, Cholesterol 24-monooxygenase, CH24H, CYP46A1, Cytochrome P450 46A1, CYP46, Cholesterol 24S-hydroxylase

Purificación

Purified by antigen affinity column.

Número de acceso

Q9Y6A2

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Cholesterol 24-hydroxylase (CYP46A1) is a ~56 kDa protein. P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation. By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning. Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis. Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain.

1. Lund, EG. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 7238-43. PMID: 10377398

2. Mast, N. et al. (2003) Biochemistry 42, 14284-92. PMID: 14640697

3. Mast, N. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 9546-51. PMID: 18621681

4. Goyal, S. et al. (2014) Journal of lipid research 55, 1933-43. PMID: 25017465

5. Bodin, K. et al. (2002) The Journal of biological chemistry 277, 31534-40. PMID: 12077124

6. Mast, N. et al. (2017) The Journal of biological chemistry 292, 4913-4924. PMID: 28190002

7. Mast, N. et al. (2010) The Journal of biological chemistry 285, 31783-95. PMID: 20667828

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human CYP46A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human CYP46A1 Polyclonal Antibody (HV358014)

Vista general

Imágenes

Referencias

Datasheet

SDS