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Anti-Human D2HGDH Polyclonal Antibody (HV254014)

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Anti-Human D2HGDH Polyclonal Antibody
Anti-Human D2HGDH Polyclonal Antibody
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Vista general
Número de catálogoHV254014
Descripción
Anti-Human D2HGDH Polyclonal Antibody (HV254014) is a rabbit polyclonal antibody detecting D2HGDH in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human D2HGDH (Arg56-Asp232).
Objetivo D2HGDH
Purificación Purified by antigen affinity column.
Número de acceso Q8N465
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosD-2-hydroxyglutarate dehydrogenase, mitochondrial, 1.1.99.39, D2HGDH, D2HGD
Fondo

D-2-hydroxyglutarate dehydrogenase, mitochondrial (D2HGDH) is a ~56 kDa protein. Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC.

1. Achouri, Y. et al. (2004) The Biochemical journal 381, 35-42. PMID: 15070399
2. Struys, EA. et al. (2005) American journal of human genetics 76, 358-60. PMID: 15609246
3. Struys, EA. et al. (2005) Annals of neurology 58, 626-30. PMID: 16037974
4. Kranendijk, M. et al. (2010) Human mutation 31, 279-83. PMID: 20020533
5. Yang, J. et al. (2021) Cell discovery 7, 3. PMID: 33431826
Nota For research use only.
Imágenes
  • Anti-Human D2HGDH Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-D2HGDH polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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