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Anti-Human DCK Polyclonal Antibody (HB117014)

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Aperçu
Numéro de catalogueHB117014
Description
Anti-Human DCK Polyclonal Antibody (HB117014) is a rabbit polyclonal antibody detecting dCK in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human DCK (Met1-Leu260).
Cible dCK, Deoxyguanosine kinase, Deoxycytidine kinase, Deoxyadenosine kinase, DCK
Purification Purified by antigen affinity column.
Numéro d'accès P27707
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Deoxycytidine kinase (DCK) is a ~30 kDa protein. Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine. Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.

1. Sabini, E. et al. (2003) Nature structural biology 10, 513-9. PMID: 12808445
2. Sabini, E. et al. (2008) Journal of molecular biology 378, 607-21. PMID: 18377927
3. Hazra, S. et al. (2009) Biochemistry 48, 1256-63. PMID: 19159229
4. Chottiner, EG. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 1531-5. PMID: 1996353
5. Hazra, S. et al. (2010) Biochemistry 49, 6784-90. PMID: 20614893
6. Smal, C. et al. (2010) Archives of biochemistry and biophysics 502, 44-52. PMID: 20637175
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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