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| カタログ番号 | HP105014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human DDX21 Polyclonal Antibody (HP105014) is a rabbit polyclonal antibody detecting DDX21 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human DDX21 (Ala187-Ile563). | ||||||||
| ターゲット | DEAD box protein 21, DDX21, Gu-alpha, Nucleolar RNA helicase II, RH II/Gu, Nucleolar RNA helicase Gu, Nucleolar RNA helicase 2 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9NR30 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Nucleolar RNA helicase 2 (DDX21) is a ~87 kDa protein. RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes. 1. Calo, E. et al. (2015) Nature 518, 249-53. PMID: 25470060 2. Song, C. et al. (2017) Genes & development 31, 1370-1381. PMID: 28790157 3. Sloan, KE. et al. (2015) Nucleic acids research 43, 553-64. PMID: 25477391 4. Westermarck, J. et al. (2002) The EMBO journal 21, 451-60. PMID: 11823437 5. Zhang, Y. et al. (2014) Breast cancer research : BCR 16, 449. PMID: 25260534 6. Valdez, BC. et al. (1997) European journal of biochemistry 250, 800-7. PMID: 9461305 9. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-DDX21 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: K562 cell lysate
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