Anti-Human DDX21 Polyclonal Antibody (HP105014)

Name: Anti-Human DDX21 Polyclonal Antibody
Brand: abinScience
SKU: HP105014
Price: 96 USD
Availability: InStock

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Каталожный номер

HP105014

Описание

Anti-Human DDX21 Polyclonal Antibody (HP105014) is a rabbit polyclonal antibody detecting DDX21 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human DDX21 (Ala187-Ile563).

Цель

DEAD box protein 21, DDX21, Gu-alpha, Nucleolar RNA helicase II, RH II/Gu, Nucleolar RNA helicase Gu, Nucleolar RNA helicase 2

Очистка

Purified by antigen affinity column.

Номер доступа

Q9NR30

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Nucleolar RNA helicase 2 (DDX21) is a ~87 kDa protein. RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes.

1. Calo, E. et al. (2015) Nature 518, 249-53. PMID: 25470060

2. Song, C. et al. (2017) Genes & development 31, 1370-1381. PMID: 28790157

3. Sloan, KE. et al. (2015) Nucleic acids research 43, 553-64. PMID: 25477391

4. Westermarck, J. et al. (2002) The EMBO journal 21, 451-60. PMID: 11823437

5. Zhang, Y. et al. (2014) Breast cancer research : BCR 16, 449. PMID: 25260534

6. Valdez, BC. et al. (1997) European journal of biochemistry 250, 800-7. PMID: 9461305

9. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-DDX21 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: K562 cell lysate

Ссылки

Даташит

Anti-Human DDX21 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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