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Anti-Human DDX46 Polyclonal Antibody (HD537014)

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Vista general
Número de catálogoHD537014
Descripción
Anti-Human DDX46 Polyclonal Antibody (HD537014) is a rabbit polyclonal antibody detecting DDX46. Suitable for Rat, Human, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human DDX46 (Asp322-Lys765).
Objetivo DDX46, DEAD box protein 46, EC:3.6.4.13, KIAA0801, PRP5 homolog, Probable ATP-dependent RNA helicase DDX46
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q7L014
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Probable ATP-dependent RNA helicase DDX46 is a ~117 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, DDX46 plays essential roles during assembly of pre-spliceosome and proofreading of the branch site.

1. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937
2. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006
3. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310
4. Yang, F. et al. (2023) Nature communications 14, 897. PMID: 36797247
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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