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Anti-Human DEFA5 Polyclonal Antibody (HX187014)

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Aperçu
Numéro de catalogueHX187014
Description
Anti-Human DEFA5 Polyclonal Antibody (HX187014) is a rabbit polyclonal antibody detecting DEFA5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human DEFA5 (Glu20-Arg94).
Cible HD5(20-94), DEF5, Defensin-5, DEFA5, Defensin, alpha 5
Purification Purified by antigen affinity column.
Numéro d'accès Q01523
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Defensin alpha 5 (DEFA5) is a ~10 kDa protein. Host-defense peptide that maintains sterility in the urogenital system. Has antimicrobial activity against a wide range of bacteria, including Gram-negative E.coli, P.aeruginosa and S.typhimurium, and Gram-positive E.aerogenes, S.aureus, B.cereus, E.faecium and L.monocytogenes. Confers resistance to intestinal infection by S.typhimurium. Exhibits antimicrobial activity against enteric commensal bacteria such as B.adolescentis, L.acidophilus, B.breve, L.fermentum, B.longum and S.thermophilus. Binds to bacterial membranes and causes membrane disintegration.

1. Ghosh, D. et al. (2002) Nature immunology 3, 583-90. PMID: 12021776
2. Salzman, NH. et al. (2003) Nature 422, 522-6. PMID: 12660734
3. Ericksen, B. et al. (2005) Antimicrobial agents and chemotherapy 49, 269-75. PMID: 15616305
4. de Leeuw, E. et al. (2009) FEBS letters 583, 2507-12. PMID: 19589339
5. Spencer, JD. et al. (2012) PloS one 7, e31712. PMID: 22359618
6. Rajabi, M. et al. (2012) The Journal of biological chemistry 287, 21615-27. PMID: 22573326
7. Schroeder, BO. et al. (2015) Mucosal immunology 8, 661-71. PMID: 25354318
8. Wang, C. et al. (2015) Journal of medicinal chemistry 58, 3083-93. PMID: 25782105
9. Ehmann, D. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 3746-3751. PMID: 30808760
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human DEFA5 Polyclonal Antibody

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