Anti-Human DGCR8 Polyclonal Antibody (HP031014)

Name: Anti-Human DGCR8 Polyclonal Antibody
Brand: abinScience
SKU: HP031014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
100ug

Número:

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Datasheet
SDS

Vista general

Número de catálogo

HP031014

Descripción

Anti-Human DGCR8 Polyclonal Antibody (HP031014) is a rabbit polyclonal antibody detecting DGCR8 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human DGCR8 (Asp436-Lys752).

Objetivo

DGCRK6, Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12

Purificación

Purified by antigen affinity column.

Número de acceso

Q8WYQ5

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Microprocessor complex subunit DGCR8 is a ~86 kDa protein. Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding.

1. Nguyen, TA. et al. (2015) Cell 161, 1374-87. PMID: 26027739

2. Kwon, SC. et al. (2016) Cell 164, 81-90. PMID: 26748718

3. Gregory, RI. et al. (2004) Nature 432, 235-40. PMID: 15531877

4. Han, J. et al. (2004) Genes & development 18, 3016-27. PMID: 15574589

5. Landthaler, M. et al. (2004) Current biology : CB 14, 2162-7. PMID: 15589161

6. Han, J. et al. (2006) Cell 125, 887-901. PMID: 16751099

7. Pauley, KM. et al. (2006) EMBO reports 7, 904-10. PMID: 16906129

8. Yeom, KH. et al. (2006) Nucleic acids research 34, 4622-9. PMID: 16963499

9. Faller, M. et al. (2007) Nature structural & molecular biology 14, 23-9. PMID: 17159994

10. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human DGCR8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo